PvdfPolyvinylidene Fluoride, Molecular Fomula: - (C0H2F2) N - Kudzivisa (kunogona kushandiswa kunze kwenguva yakareba), radiation kuramba, Dizoelectric zvivakwa, chimiro cheFluoroccarbon, hydrogen enture inoumba zvakanyanya uye yakasimba musanganiswa.Pvdfine hurombo hydrophiciticity.

Pvdfmembrane ndeye hydrophobic membrane usati warapwa, mushure mekurapwa kwemakumi methanol,Pvdfmembrane anova hydrophilic. Iwe unofanirwa kunge wakamboona izvi mukuyedza.

Saka, chero bedziPvdfmembrane inobatwa nemethanol kwemakumi matatu masekondi kana zvakadaro, iyoPvdfmembrane inogona kushandurwa zvachose kubva ku hydrophobic mamiriro ku hydrophilic, uye mhedzisiro yacho yakafanana mushure mekuwedzera kwenguva. Panguva imwecheteyo, tarisa nemaziso asina kupfeka ingova nenzvimbo chena - senzvimbo kana nzvimbo dzekuvharira paMembrane pamusoro, uye wozonyudza membrane muTramisbuffer ye15min. The thePvdfmembrane inoshandiswa yakanyudzwa mu methanol ye 1 - 2min. Iyo membrane yekuraira bhuku rekambani rinoti yakanyudzwa mu methanol ye 1 - 2min.

PvdfMembrane anogona kusunga mapuroteni uye akaparadzanisa zvidimbu zvidiki zvemapuroteni. Yakatangwa yakatanga kushandiswa protein kutevedzana. Sezvo nitrocellulose membrane ingashamba muEdman Reagent,Pvdfyakatsvaga seyinotsiva, kunyangwe iyo yekubudirira kwePvdfMembrane inosunga mapuroteni akange asina kukwirira seNitrocellulose membrane. Asi kugadzikana kwayo uye kupokana kwekukanganisa kunoita kuti ive yakakodzera kutevedzerwa puroteni, uye ichiramba ichishandiswa nhasi.PvdfMembrane uye nitrocellulose membrane, inogona kuve akasiyana-siyana emakwenzi uye repemiluminescence yekuona, asi zvakare ine huwandu hwakawanda hwekushandisa. IzviPvdfMembrane ane sensitivity yakakwirira, resolution uye protein yekubatana pane membrames pasi pehunyanzvi hwekusagadzikana, ichiita kuti ive yakakodzera kuwanikwa kwemapuroteni ehuremu akaderera.

Nekudaro, usati washandisaPvdfMembrane, inofanira kunyeverwa nemakumi mana nemakumi mana emamita, uyezve zvakaringana mukutamaniswa zvisati zvaitika zvisati zvaitika (chinangwa che methanol bubble inPvdfmembrane ndeyekuita kuti tishandise boka rakanaka panePvdfmembrane, kuitira kuti zviri nyore kusanganisa neyekukasira mapuroteni. Iyo inotamisa iyo isina methanol inogona kushandiswa mukuendeswa kwekunyepedzeraPvdfmembrane.

SDS inowedzera iyo solubility yemvura yeProtein uye inosimudzira protein yekushambira mumhinduro yemagetsi. Kana pasina maSD anowedzerwa, mapuroteni anozivisa pamusoro peglue, asi zvidhinha zvakawandisa zvichakanganisa adsorption yemapuroteni neMemo.

Methanol anogona kupatsanura zvidyo kubva protein, uye nekukasira kusunganidzwa kweMethanol, nekukasira kuparadzaniswa kweSDS kubva protein, asi isingakwanisi kupuranyira protein iri kupera kubuda muglue. Kazhinji kutaura, kusunganidzwa kwe methanol ndeye 20%, asi kugona kwePVDF membrane yeMethinol kune methanol kusvika ku25% (Iine pesvedzero shoma paMembrane kufambisa kwemapuroteni, uye kufungidzira kwehunyanzvi kuri kuwedzera). Kutamiswa kukuru kweProtein kunogona kutariswa kuderedza methanol kusangana, mukuwedzera zvidhinha zvinofanirwa kuwedzerwa. Rimwe basa re methanol ndere kutonhorera pasi. Nekuda kwekushandiswa kweElectrolyte uye kulatlilization yeMethanol, iyo inochinja kana voltage inochinja nekukurumidza uye tembiricha inokwira nekukurumidza mune yekare electroconSendsion, saka imwe methanol inogona kutariswa. Uku ndiko kukosha kukuru kune iyo semi