Pvdfpolyvinylidene fluoride, molecular mis: - (c2h2f2) n - Tawm sab nraud ntev ntev), hluav taws xob tiv thaiv, cov qauv siv tshuaj lom zem, cov qauv siv tshuaj lom neeg ua ke thiab cov khoom siv tsis sib haum xeeb tshaj plaws.Pvdfmuaj tsis zoo hydrophilicity.

Pvdfmembrane yog hydrophobic membrane ua ntej kev kho mob methanol,Pvdfmembrane los ua hydrophilic. Koj yuav tsum tau pom qhov no hauv kev sim.

Yog li ntawd, ntev npaum li covPvdfmembrane yog kho nrog methanol rau 30 vib nas this lossis yog li ntawd, tusPvdfmembrane tuaj yeem hloov kho tag nrho los ntawm hydrophobic xeev rau hydrophilic, thiab cov nyhuv yog tib yam tom qab txuas ntxiv ntawm lub sijhawm. Nyob rau tib lub sijhawm, saib nrog cov qhov muag liab qab seb puas muaj cov chaw dawb - li ntawm daim nyias nyias qab hau, thiab tom qab ntawd cov ntaub qhwv caj dab rau hauv lub tsheb xa khoom rau 15min. TusPvdfmembrane siv tau tsau rau hauv methanol rau 1 - 2min. Daim Ntawv Qhia Txog Daim Ntawv Qhia ntawm lub tuam txhab hais tias nws yog soaked hauv methanol rau 1 - 2min.

Pvdfmembrane tuaj yeem khi cov protein thiab cais cov nqaij tawm me me. Nws tau pib siv rau cov protein exctricect. Txij li thaum nitrocellulose membrane yuav txwv nyob rau hauv Edman Reagent,Pvdftau nrhiav ua hloov chaw, txawm hais tias qhov ua tau zoo ntawmPvdfMembrane khi cov protein tsis yog siab li nitrocellose membrane. Tab sis nws ruaj khov thiab corrosion tsis kam ua rau nws zoo tagnrho rau cov protein ua ntu zus, thiab nws tseem nyob hauv kev siv hnub no.Pvdfmembrane thiab nitrocellulose membrane, tuaj yeem yog ntau yam ntawm staining thiab chicumuminescence nrhiav, tab sis kuj tseem muaj ntau daim ntawv thov. NoPvdfMembrane muaj cov kev xav tau siab dua thiab muaj kev daws teeb meem thiab cov muaj protein ntau dua li cov tshuaj uas muaj zog tsis zoo ntawm cov protein tsawg.

Txawm li cas los xij, ua ntej sivPvdfmembrane, nws yuav tsum tau ua pethanol, thiab tom qab ntawd sib npaug hauv tus xa khoom ua ntej nws siv tau (lub hom phiaj ntawm methanol npuas hauvPvdfmembrane yog kom qhib cov pab pawg zoo rauPvdfmembrane, kom nws yooj yim dua los ua ke nrog cov tshuaj tiv thaiv tsis zoo). Tus hloov khoom tsis muaj methanol tuaj yeem siv rau hauv kev hloov pauv ntawm pretreatedPvdfmembrane.

SDS nce cov dej solubility ntawm cov protein thiab txhawb cov protein ua luam dej hauv electrolyraphy daws. Yog tias tsis muaj SDS ntxiv, protein yuav yooj yim rau cov kua nplaum, tab sis SDS ntau dhau yuav cuam tshuam cov protein thiab membrane.

Methanol tuaj yeem cais cov SDs los ntawm cov protein los ntawm cov protein, tab sis lub siab sib cais ntawm cov protein, tab sis lub siab kub ntawm cov protein, uas tsis muaj txiaj ntsig zoo rau cov protein khiav tawm ntawm cov kua nplaum. Feem ntau hais lus, lub concentration ntawm methanol yog 20%, tab sis muaj peev xwm suav tau tias muaj kev cuam tshuam me me ntawm kev hloov pauv ntawm daim ntawv me me ntawm cov protein ntau, thiab cov xim sib cuam tshuam yog ntau dua). Kev hloov protein loj tuaj yeem txiav txim siab los txo methanil concentration, ntxiv rau SDS yuav tsum tau ntxiv. Lwm txoj haujlwm ntawm methanol yog kom txias. Vim tias kev noj ntawm hluav taws xob electrolyte thiab volatilization hloov sai dua hauv cov qub electroconversion kua thaum electroconversion, yog li ntxiv Methanol tuaj yeem txiav txim siab. Qhov no yog qhov tseem ceeb rau lub semi - qhuav hloov chaw tsis huv, vim tias kev noj ntawm semi -